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Opinions...


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Posted by Sundberg on September 24, 2001 at 00:26:03:

In Reply to: Re: RFLPs & AFLPs posted by Eric on September 23, 2001 at 22:13:02:

: I would follow the paper, if it were written in a peer reviewed journal (J. herp, Herpetologica, Systematic Biology etc.) These methods are commonly used for systematics, and if used correctly should be considered good tools. All data have their limitations though. To what paper are you referring?

Thanks for the response. The paper I'm referring to is;
Giannasi N, Thorpe RS, Malhotra A.
The use of amplified fragment length polymorphism in determining species trees at fine taxonomic levels: analysis of a medically important snake, Trimeresurus albolabris.
Molecular Ecology, February 2001.

Indeed, a prominent herpetologist I've been in contact with recommends following the paper. Usually I wouldn't hesitate to follow advice from that source. Unfortunately, another academic person, who is a geneticist and working with the classification of reptiles, doesn't feel that RFLPs & AFLPs are adequate evidence for boundaries at the (sub-) species level... Thus my confusion. I would like to hear the opinions of some people who is more acquinted with the status of these methods than I am.

All the best,

Sundberg

surevsaha at hotmail dot com



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