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Re: RFLPs & AFLPs


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Posted by Eric on September 23, 2001 at 22:13:02:

In Reply to: RFLPs & AFLPs posted by Sundberg on September 19, 2001 at 00:44:49:

: There are a couple of methods used for determining (sub-) species boundaries that I'd like to know what people "in-the-know" think of. I'm talking about RFLPs (restriction fragment length polymorphisms) and AFLPs (amplified fragment length polymorphisms).

: I have heard contradictionary opinions regarding these methods. Unfortunately I do not have the necessary knowledgde to come to my own conclusion on the issue, which is why I'd like to hear more opinions on them. I'm currently in the progress of writing about a particular genus, and there is a recent paper elevating some of the subspecies to full species status based on AFLPs. Need to decide if I am to follow this paper or not.

: /Sundberg

: surevsaha at hotmail dot com

I would follow the paper, if it were written in a peer reviewed journal (J. herp, Herpetologica, Systematic Biology etc.) These methods are commonly used for systematics, and if used correctly should be considered good tools. All data have their limitations though. To what paper are you referring?


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